Mistakes when loading data

1

when I load the data, there are some mistakes, what can I do.
Error uploading sample seRNA-seq. Invalid matrix.mtx file: Matrix is transposed. Invalid barcodes.tsv file: 60662 barcodes expected, but 58197 found. Invalid features/genes.tsv file: 58197 genes expected, but 60662 found.
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2

Hi, it looks like the issue you’re encountering is due to the matrix being transposed, which means the rows and columns are swapped.

I’d suggest reading the matrix in R Studio, transposing it, and then exporting it. Here’s how you can do this:

  • Read the matrix:
library(Matrix)
matrix <- readMM("path_to_your/matrix.mtx")
  • Transpose the matrix:
transposed_matrix <- t(matrix)
  • Export the transposed matrix:
writeMM(transposed_matrix, "path_to_your/transposed_matrix.mtx")

I hope this helps.

3

thank you very much. I got it

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